biotinylate

English

Etymology

biotinyl +‎ -ate

Verb

biotinylate (third-person singular simple present biotinylates, present participle biotinylating, simple past and past participle biotinylated)

1. To attach a biotin residue to a biological macromolecule in order to label it
   • 2015 July 11, Eugene J. H. Wee, Thu Ha Ngo, Matt Trau, “Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs”, in Clinical Epigenetics‎^[1], volume 7, →DOI:

     After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.

Related terms

• biotinylation