Definition from Wiktionary, the free dictionary
Formed through analogy with the effect of a shotgun blast, which contains many small pellets.
- (genetics) A DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and reassembled to form a sequence of the original strand.
2005, Selene K Swanson; Michael P. Washburn, “The continuing evolution of shotgun proteomics”, Drug Discovery Today, page 719:
- In shotgun sequencing of genomic DNA, fragments of a genome are sequenced and computationally reassembled to determine the genome of an organism