polycloning
Jump to navigation
Jump to search
English
[edit]Etymology
[edit]Adjective
[edit]polycloning (not comparable)
- Relating to the production of multiple types of cloning
- 2015 November 26, “Comprehensive Survey of miRNA-mRNA Interactions Reveals That Ccr7 and Cd247 (CD3 zeta) are Posttranscriptionally Controlled in Pancreas Infiltrating T Lymphocytes of Non-Obese Diabetic (NOD) Mice”, in PLOS ONE[1], :
- The oligonucleotides were annealed and cloned into the pmirGLO vector (Promega Corporation, USA) between the XhoI/XbaI restriction sites of the polycloning site (PCS), resulting in the miRNA target region in the correct 5´ to 3´ orientation and immediately downstream of the luciferase gene.