2016 February 12, “Circulating Hepcidin-25 Is Reduced by Endogenous Estrogen in Humans”, in PLOS ONE[1], DOI:10.1371/journal.pone.0148802:
Pr ) ELISA was performed by using streptavidin-coated and blocked wells that had been incubated for 1 h with biotinylated antihepcidin capture antibody (2 mg/L).