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phenylmethylsulfonylfluoride (uncountable)

  1. (biochemistry) A serine protease inhibitor commonly used in the preparation of cell lysates.
    • 2015 June 5, Jun Chai et al., “Effects of sevoflurane on tight junction protein expression and PKC-α translocation after pulmonary ischemia–reperfusion injury”, in Experimental & Molecular Medicine[1], volume 47, DOI:10.1038/emm.2015.27:
      One hundred milligrams of frozen lung tissue was homogenized in buffer A (0.32 M sucrose, 5 m M Tris–HCl pH 7.5, 120 m M KCl, 1 m M EDTA, 0.2 m M phenylmethylsulfonylfluoride, 1 μg ml −1 leupeptin, 1 μg ml −1 pepstatin and 1 μg ml −1 aprotinin) and spun at 4 °C, 12 890  g for 10 min.